Journal: Skin Research and Technology

Article Title: Ghrelin induced by ultraviolet B exposure promotes the restoration of diabetic cutaneous wound healing

doi: 10.1111/srt.13919

Figure Lengend Snippet: Ghrelin protects human umbilical vein endothelial cells (HUVECs) against high glucose‐induced injury on cell migratory and angiogenic activities. Cells were added with 33 mM glucose with or without ghrelin treatment. After treatment, wound healing assay (A) showcasing the migration distance of HUVECs at 6 and 12 h post‐wounding. Tube formation assay (B) were conducted to highlight the number of branch points of treated cells. The protein expression (C) and secreted level (D) of vascular endothelial growth factor were determined by the western blot analysis and ELISA, respectively. Data were presented as mean ± SEM. *** p < 0.001; # p < 0.05, ## p < 0.01, and ### p < 0.001.

Article Snippet: The human umbilical vein endothelial cells (HUVECs; iCell‐h110) were purchased for iCell Research Institute (Bangkok, Thailand) and maintained in endothelial cell growth medium‐2 (EGM‐2) supplemented with the EGM‐2 BulletKit (Lonza Group Ltd, Basel, Switzerland) at 37°C with 5% CO2.

Techniques: Wound Healing Assay, Migration, Tube Formation Assay, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Brain

Article Title: Gene-mapping study of extremes of cerebral small vessel disease reveals TRIM47 as a strong candidate

doi: 10.1093/brain/awab432

Figure Lengend Snippet: TRIM47 depletion in human brain endothelial cells increases endothelial permeability. ( A ) Efficient reduction of TRIM47 expression in endothelial cells was achieved by siTRIM47 #1 and 2 versus siControl. Ve-cadherin expression, a marker of endothelial cell junction was followed and α tubulin was used as a loading control. ( B ) Endothelial transwell permeability assay. Quantification of dextran leakage expressed as fold change compared to HBMEC and ( C ) hCMEC/D3 ( D ) cells treated with control siRNA TRIM47 #1 and #2 versus siControl. Fluorescent intensity was normalized to siControl knock down. Two-way ANOVA with a Dunnett’s multiple comparisons test was performed. Data are presented as mean ± SD. ** P -value < 0.01, *** P < 0.0001, *** P > 0.00001.

Article Snippet: HBMEC (human brain microvascular endothelial cells; Alphabioregen-Clinisciences, #ALHE02) were grown in endothelial basal medium-2 (EBM-2) supplemented with EGM-2 BulletKits (Lonza #CC-3162) with 0.1% gelatin coating.

Techniques: Permeability, Expressing, Marker, Control, Knockdown